| A Quick and Safe Method for Fungal DNA Extraction |
|
Myoung Hwan Chi, Sook Young Park, Yong Hwan Lee |
|
|
| Abstract |
|
DNA-based studies, including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a simple electric grinder. This QS method is applicable not only to a broad range of microbial eukaryotes, including true fungi and oomycetes, but also to lichens and plants. |
| Key Words:
fungi, genomic DNA extraction, high-throughput |
|
PDF Links
Full text via DOI
Download Citation
