Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of Apricot

Li, Bin; Fang,  Yuan; Zhang, Guo Qing; Yu, Rong Rong; Lou, Miao Miao; Xie, Guan Lin; Wang, Yan Li; Sun, Guo Chang

doi:2010.26.3.223

Plant Pathol J > Volume 26(3); 2010 > Article

The Plant Pathology Journal 2010;26(3):223-230.
DOI: https://doi.org/10.5423/PPJ.2010.26.3.223 Published online September 30, 2010.

Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of Apricot

Bin Li, Yuan Fang, Guo Qing Zhang, Rong Rong Yu, Miao Miao Lou, Guan Lin Xie, Yan Li Wang, Guo Chang Sun

Abstract

The Burkholderia cepacia complex isolates causing bacterial fruit rot of apricot were characterized by speciesspecific PCR tests, recA-HaeIII restriction fragment length polymorphism (RFLP) assays, rep-PCR genomic fingerprinting, recA gene sequencing, and multilocus sequence typing (MLST) analysis. Results indicated that the isolates Bca 0901 and Bca 0902 gave positive amplifications with primers specific for B. vietnamiensis while the two bacterial isolates showed different recARFLP and rep-PCR profiles from those of B. vietnamiensis strains. In addition, the two bacterial isolates had a higher proteolytic activity compared with that of the non-pathogenic B. vietnamiensis strains while no cblA and esmR marker genes were detected for the two bacterial isolates and B. vietnamiensis strains. The two bacterial isolates were identified as Burkholderia seminalis based on recA gene sequence analysis and MLST analysis. Overall, this is the first characterization of B. seminalis that cause bacterial fruit rot of apricot.

Key Words: apricot fruit rot, Burkholderia cepacia complex, Burkholderia seminalis, Burkholderia vietnamiensis, identification