The Plant Pathology Journal 2012;28(3):299-305.
Published online September 30, 2012.
Research Notes : Characterization of a Novel Cucumber mosaic virus Isolated from Petunia hybrida
Kyung Sook Han, Gug Seoun Choi, Bong Nam Chung, Jeom Duk Cho, In Sook Cho, Kee Hong Kim, Su Kim, Ju Yeon Yoon, Seung Kook Choi
Abstract
Petunia hybrida is commonly used in landscapes and interiors for its attractive flower. Virus-like foliar symptoms, including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins, were observed on a petunia plant from Icheon, Gyeonggido, Korea. Cucumber mosaic virus (CMV) was identified in the symptomatic petunia by serological testing for the presence of CMV coat protein (CP) with a direct antibody-sandwich-enzyme-linked immunosorbent assay. An agent was mechanically transmitted to indicator plant species including Chenopodium quinoa. Examination of the inoculated plant leaves by RT-PCR analysis and electron microscopy revealed the presence of specifically amplified CP products and spherical virions of approximately 28 nm in diameter, respectively, providing confirmation of a CMV infection. Analysis of CP sequences showed that CMV petunia isolate (CMVYJC) shared 82.5-100% amino acid sequence identity with CPs of representative CMV strains. Phylogenetic analysis of CPs supports that CMV-YJC is a member of CMV subgroup IA (CMV-IA) and has biological properties of CMV-IA on host species. To our knowledge, this is the first report of CMV from P. hybrida in Korea.
Key Words: coat protein, Cucumber mosaic virus, petunia, RT-PCR, sequence analysis, symptom


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