Plant Pathol J > Volume 28(4); 2012 > Article
The Plant Pathology Journal 2012;28(4):423-427.
DOI: https://doi.org/10.5423/PPJ.NT.05.2012.0061    Published online December 31, 2012.
Occurrence of a New Type of Pseudomonas syringae pv. actinidiae Strain of Bacterial Canker on Kiwifruit in Korea
Young Jin Koh, Gyoung Hee Kim, Hyun Seok Koh, Young Sun Lee, Seong Cheol Kim, Jae Sung Jung
Abstract
Pseudomonas syringae pv. actinidiae strains, the causal agents of bacterial canker on kiwifruit, were isolated from Korea and Italy in 2011. Among 87 isolates, a total of six representative strains, three from Korea and three from Italy, were identified on the basis of biochemical and physiological tests. Identities were confirmed by PCR using P. syringae pv. actinidiae-specific primers PsaF1/R2, which amplified a 280-bp DNA fragment. The strains isolated from Korea in this study displayed BOX-PCR patterns similar to those isolated from Italy but different from those isolated previously in Korea or the pathotype P. syringae pv. actinidiae strain. The effector hopA1 and hopH1 genes, which are known to be present in strains isolated recently from France and Italy, were also present in P. syringae pv. actinidiae strains, SYS1, SYS2 and SYS4, isolated from Korea in this work. However, no amplicons of the expected size were obtained from strains previously isolated from Korea and Japan. In addition, the Korean strains isolated in this work belonged to haplotype I for the cts gene identical to those strains isolated from recent outbreaks in Italy. These results suggest that P. syringae pv. actinidiae strains isolated from Korea and examined in this work are a new type of strain similar to those found from recent outbreaks in Italy. This is the first report on the occurrence of cts haplotype I strains of P. syringae pv. actinidiae affecting kiwifruit plants in Korea.
Key Words: bacterial canker, BOX-PCR, hopA1, hopH1, kiwifruit, Pseudomonas syringae pv. actinidiae


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