Plant Pathol J > Volume 36(2); 2020 > Article
Kim, Kwon, Bae, and Kwak: Proteomic Reference Map and Comparative Analysis between Streptomyces griseus S4-7 and wbiE2 Transcription Factor-Mutant Strain


Streptomyces griseus S4-7, a well-characterized keystone taxon among strawberry microbial communities, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the present study, we created a proteomics reference map for S. griseus S4-7. Additionally, analysis of differentially expressed proteins was performed against a wblE2 mutant, which was deficient in spore chain development and did not express an antifungal activity-regulatory transcription factor. We believe that our data provide a foundation for further in-depth studies of functional keystone taxa of the phytobiome and elucidation of the mechanisms underlying plant-microbe interactions, es-pecially those involving the Streptomyces genus.

Suppressive soils are defined as “soils in which the pathogen does not establish or persist, establishes but causes little or no damage, or establishes and causes disease for a while but thereafter the disease is less important, although the pathogen may persist in the soil” (Weller, 2007). In contrast, the disease in question readily occurs and persists in non-suppressive soils (conducive soils). Currently, many different types of suppressive soils have been reported, including those with suppressiveness against Fusarium oxsporum, Streptomyces scabies, and Heterodera avenae (Weller et al., 2002). Fusarium wilt of strawberry is caused by F. oxysporum f. sp. Fragariae, which forms three types of spores: microconidia, macroconidia, and chlamydospores. Chlamydospores can persist in a dormant stage in the soil for as long as 30 years, and each of these spores can be spread through running water, farm implements, and machinery (Couteaudier and Alabouvette, 1990). Reliable chemical control of strawberry Fusarium wilt disease is unavailable to date. Streptomyces griseus S4-7 has been isolated from a strawberry field and characterized as the suppressive agent in Fusarium wilt in a previous study (Cha et al., 2016). Interestingly, the strain was detected in the strawberry flower as well as in the pollinator body (Kim et al., 2019a). Streptomyces griseus S4-7 is unnoted the most of basic information such as antibiotic biosynthesis pathway, regulatory mechanisms (Kim et al., 2019b). Previous proteomic studies of Streptomyces spp. focused on membrane proteome analysis and gene mapping for substance analysis. The wblE2 mutant, deficient in a whi-type transcription factor, failed to inhibit the growth of the fungal pathogen or confer plant protection (Cho et al., 2017). In the present study, we aimed to create a proteomic reference map of the S4-7 strain. Proteomic mapping of S4-7 should provide insights into S4-7 physiology and enable investigation of its potential for antibiotic biosynthesis and biocontrol applications.
Bacterial strains, S4-7 and wblE2 mutant, were cultured in 25 ml of YEME (yeast extract 3 g, malt extract 3 g, peptone 3 g, glucose 10 g, sucrose 170 g, and agar 20 g per liter). The cells were harvested by centrifugation and resuspended as 108 colony-forming units/ml. Then, 1 ml of the bacterial solution was inoculated onto nitrocellulose membranes coated with MS (mannitol soya flour agar: 20 g of mannitol, 20 g of soya flour, and 20 g of agar per 1 liter). The mycelium was scraped from the cellophane discs at three different time points during colony development for further observation: at 24 h, when only vegetative mycelium was observed; at 48 h, when aerial mycelium covered the plates; and at 72 h, when the color of the mycelium surface turned gray because of sporulation (Supplementary Fig. 1).
Proteins were extracted from the S4-7 and ΔwblE2 strains at three developmental time points (24, 48, and 72 h) using the trichloroacetic acid/acetone/phenol extraction method (Kwon et al., 2014). The extracted proteins were quantified using a 2D-Quant Kit (Amersham Biosciences, Bukinghamshire, UK). Soluble protein (300 µg) was used for two-dimensional gel electrophoresis (2-DE) analysis. For isoelectric focusing, PROTEAN IEF cell (Bio-Rad, Hercules, CA, USA) was used with immobilized pH gradient strips (17 cm, pH 4-7, Bio-Rad). Then, two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed using the Protein Xi-II Cell System (Bio-Rad). The gels were stained with Colloidal Coomassie Blue G-250 (Candiano et al., 2004). Images were acquired using a GS-800 Imaging Densitometer (Bio-Rad). Abundant proteins were detected with PDQUEST (version 7.2.0, Bio-Rad). Spot densities were normalized to a relative density, and the mean values from triplicates were compared. The cut-off for differential expression was set at 1.5-fold change. To identify abundant proteins, the spots were excised and digested by in-gel tryptic digestion. The cleavage (peptide) solution was loaded onto a matrix-assisted laser desorption inonization-time of flight/mass spectrometry (MALDI-TOF/MS) sample plate and analyzed by an ABI 4800 Plus TOF-TOF mass spectrometer (Applied Biosystems, Foster City, CA, USA). MALDI-TOF MS spectra were queried using the National Center for Biotechnology Information (NCBI) protein database with ProteinPilot (version 3.0, AB Sciex, Framingham, MA, USA) and the MASCOT search engine (version 2.3.02, Matrix Science, London, UK). The statistical significance threshold was set at P < 0.05.
In S. griseus, a total of 435 protein spots were detected from the 2-DE gel (Fig. 1) and 223 proteins were identified and assigned functions (Supplementary Table 1). Functional distribution of the identified proteins was as follows: protein biosynthesis (16%), oxidoreductase activity (9%), transcription and regulation (9%), hydrolase activity (6%), protein folding (5%), transferase activity (5%), glucose metabolism process (5%), ATP synthesis (5%), amino acid biosynthesis, DNA damage and repair, response to stress, transport, ligase, tricarboxylic acid cycle and inositol biosynthesis process (2% each), hypothetical protein (11%), and uncharacterized protein (11%) (Fig. 2). The data indicated that the strain used energy sources mainly for primary metabolism, and that the strain required large amounts of amino acids and related metabolic processes for development and growth under laboratory culture conditions.
In total, 41 proteins were detected as difference in abundance proteins in the wild-type S4-7 and ΔwblE2 strains (Table 1, Fig. 3). All the detected proteins were identified as proteins of Streptomyces origin after database searching. At 24 h, only six proteins were showed a difference in abundance. In contrast, at 48 h and 72 h, 33 and 34 proteins, respectively, were identified as significantly regulated by the whi transcription factor. Among the difference abundant proteins, 13 were consistently less abundance in the mutant strain. Among the carbohydrate metabolism proteins, two were less abundant proteins and the other two were more abundant proteins. Most of the identified stress response, transport, DNA repair, and amino acid metabolism proteins were more abundant in the ΔwblE2 strain. Interestingly, five transcription-related proteins were detected as difference in abundance, and four of them were significantly less abundant proteins. The four less abundant transcription-related proteins were identified as the cyclic AMP receptor protein/fumarate-nitrate-reductase (crp/fnr) family transcriptional regulator, which is the key transcription factor in antibiotic production in Actinomycetes. The function of the crp/fnr transcription factor, similar to that of whi transcription factors, is known to involve regulation of colony development and spore germination in Streptomyces (Derouaux et al., 2004). Recently, a crp/fnr family transcriptional regulator was described as a global regulator of antibiotic production in Streptomyces (Gao et al., 2012). In our proteomic analyses, crp/fnr genes were significantly less abundant proteins in the Δwhi strain relative to the wild-type strain (Table 1). These results indicate that (1) the whi transcription factor regulates antibiotic production associated with the plant-protective ability of the S4-7 strain in the strawberry rhizosphere; and (2) this transcription factor may act upstream of the crp/fnr genes during colony development and secondary metabolite production. A typical phenotype of the Δcrp comprises reduced colony development and germination, but accelerated sporulation (Derouaux et al., 2004; Gao et al., 2012). By contrast, morphological characteristics of the ΔwblE2 included decreased spore and aerial hypha development.
In conclusion, the S. griseus S4-7 strain has exceptional ability to suppress the growth of F. oxysporum f. sp. fragariae; however, the underlying antibiotic biosynthesis pathways and regulatory mechanisms remain to be elucidated. In this study, proteomics mapping of S. griseus S4-7 proteins provided novel insights into these aspects (Fig. 4). All 435 proteins of the strain were observed; among them, 223 proteins were identified. Additional study of these functions is warranted. The present findings establish a foundation to explore the potential of the S. griseus S4-7 strain as a new biocontrol agent.

Electronic Supplementary Material

Supplementary materials are available at The Plant Pathology Journal website(


This research was supported by the Next-Generation Bio-Green21 Program (PJ013250).


Candiano, G., Bruschi, M., Musante, L., Santucci, L., Ghiggeri, G. M., Carnemolla, B., Orecchia, P., Zardi, L. and Righetti, P. G. 2004. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis. 9:1327-1333.
Cha, J.-Y., Han, S., Hong, H.-J., Cho, H., Kim, D., Kwon, Y., Kwon, S.-K., Crüsemann, M., Lee, Y. B., Kim, J. F., Giaever, G., Nislow, C., Moore, B. S., Thomashow, L. S., Weller, D. M. and Kwak, Y.-S. 2016. Microbial and biochemical basis of Fusarium wilt-suppressive soil. ISME J. 10:119-129.
Cho, H. J., Kwon, Y. S., Kim, D.-R., Cho, G., Hong, S. W., Bae, D.-W. and Kwak, Y.-S. 2017. wblE2 transcription factor in Streptomyces griseus S4-7 plays an important role in plant protection. MicrobiologyOpen. 6:e00494.
crossref pmc
Couteaudier, Y. and Alabouvette, C. 1990. Survival and inoculum potential of conidia and chlamydospores of Fusarium oxysporum f. sp. lini in soil. Can. J. Microbiol. 36:551-556.
crossref pmid
Derouaux, A., Dehareng, D., Lecocq, E., Halici, S., Nothaft, H., Giannotta, F., Moutzourelis, G., Dusart, J., Deveese, B., Titgemeyer, F., Van Beeuman, J. and Rigali, S. 2004. crp of Streptomyces coelicolor is the third transcription factor of the large CRP-FNR superfamily able to bind cAMP. Biochem. Biophy. Res. Commun. 325:983-990.
Gao, C., Mulder, D. and Yin, C. 2012. Crp is a global regulator of antibiotic production in Streptomyces. mBio. 6:e00407-12.
Kim, D.-R., Cho, G., Jeon, C.-H., Weller, D. W., Thomashow, L. S., Paulitz, T. C. and Kwak, Y.-S. 2019a. A mutualistic interaction between Streptomyces bacteria, strawberry plants and pollinating bees. Nat. Commun. 10:4802
Kim, D.-R., Jeon, C.-H., Shin, J.-H., Weller, D. M., Thomashow, L. and Kwak, Y.-S. 2019b. Function and distribution of lantipeptide in strawberry Fusarium wilt disease-suppressive soils. Mol. Plant-Microbe Interact. 32:306-312.
Kwon, Y. S., Kim, S. G., Chung, W. S., Bae, H., Jeong, S. W., Shin, S. C., Jeong, M.-J., Park, S.-C., Kwak, Y.-S., Bae, D.-W. and Lee, Y. B. 2014. Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation. Fungal Biol. 118:433-443.
Weller, D. M. 2007. Pseudomonas biocontrol agents of soilborne pathogens: looking back over 30 years. Phytopathology. 97:250-256.
crossref pmid
Weller, D. M., Raaijmakers, J. M., McSpadden Gardener, B. B. and Thomashow, L. S. 2002. Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu. Rev. Phytopathol. 40:309-348.
crossref pmid
Fig. 1
Protein expression profile of Streptomyces griseus S4-7. Proteins loaded onto 17 cm isoelectric focusing strips, pH 4 to 7 linear gradient. The strip was placed on top of 12.5% polyacrylamide gels for sodium dodecyl sulfate polyacrylamide gel electrophoresis. The gel was stained with Coomassie Brilliant Blue R-250.
Fig. 2
Functional classification of identified proteins in Streptomyces griseus S4-7.
Fig. 3
Representative two-dimensional gel electrophoresis image of the S4-7 and wblE2 mutant at different developmental stages. A total of 300 μg of soluble protein was loaded and separated using an immobilized pH gradient strip (17 cm, pH 4-7) and 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis. The numbers on the gels showed differentially expressed proteins between the S4-7 and the whiE2 mutant.
Fig. 4
A possible model for the mechanism of action of whi transcription factor in antibiotic production and regulation of Streptomyces griseus S4-7.
Table 1
List of difference abundant proteins in the S4-7 and wblE2 mutant during developmental stages
No. Protein ID (UniProt) MW/pI, theor. SC (%) Scorea MP Peptide hitb Fold change Biological function

S4-7 vs. wblE2

24 h 48 h 72 h
1 10 kDa chaperonin B1W3U3 10,983/4.81 65 246 5 4 -1.62 +2.45 +1.86 Protein folding
2 50S ribosomal protein L29 S2Y924 8,423/6.59 39 75 4 2 - +1.58 +1.60 Translation
3 Uncharacterized protein Q9RJ72 14,528/4.67 45 41 5 0 - -2.96 -2.54 Unknown
4 Glyoxalase V6KE74 16,023/4.98 62 48 6 0 - - +3.26 Stress response
5 Peptidyl-prolyl cis-trans isomerase Q53IB1 17,782/6.29 66 218 9 4 - +2.33 +1.92 Protein folding
6 Transcriptional regulator, CarD family G0PWJ3 17,818/5.54 62 255 7 5 - +1.82 +1.65 Transcription
7 GNAT family toxin-antitoxin system, toxin component D9WA37 20,319/6.05 41 42 4 0 - +2.05 +1.67 Stress responsesubunit
8 ATP-dependent Clp protease proteolytic G2NHF8 21,617/4.58 47 154 9 4 - - +2.24 Stress response
9 Single-stranded DNA-binding protein B4V9L5 20,053/5.29 37 240 7 4 - +2.47 +1.88 DNA replication
10 Ribosome-recycling factor G0Q1P2 20,904/5.46 48 444 16 8 - - +5.49 Translation
11 Crp/Fnr family transcriptional regulator A0A087KFI7 51,508/5.23 19 149 11 4 - -3.35 -3.71 Transcription
12 Crp/Fnr family transcriptional regulator A0A087KFI7 51,508/5.23 22 339 13 7 - -6.23 -7.34 Transcription
13 50S ribosomal protein L25 W9FNI6 20,450/4.86 23 114 4 2 -2.49 - - Translation
14 Proteasome subunit beta V6UE24 30,176/4.85 54 520 16 8 - -2.11 -1.92 Protein degradation/pathogenesis
15 Elongation factor Tu A6N2C1 36,111/5.01 34 405 9 5 -1.63 -12.34 -10.78 Translation
16 Uracil-DNA glycosylase W9FK54 25,330/5.71 47 144 9 4 - +2.65 +3.20 DNA damage/DNA repair
17 Crp/Fnr family transcriptional regulator A0A087KFI7 51,508/5.23 33 427 14 7 - -3.49 -4.22 Transcription
18 Chlorite dismutase W9FXT2 27845/5.90 68 491 16 8 - +1.96 - Stress response
19 Lipoprotein M3FS09 30955/5.06 31 38 6 0 +2.44 - - Transport/Virulence
20 Electron transfer flavoprotein subunit alpha A0A087JXW9 32481/4.97 44 666 8 8 - +3.13 +1.95 Transport
21 Proteasome subunit alpha G0Q7E7 29503/4.83 35 222 9 4 -3.97 - -2.68 Protein degradation
22 Methyltransferase A0A087K8E5 32599/6.37 70 619 19 7 - +4.72 +3.51 Stress response
23 Cysteine desulfurase A0A087KB50 27328/5.25 68 303 14 5 - +2.96 +2.04 Transport
24 Crp/Fnr family transcriptional regulator A0A087KFI7 51508/5.23 33 533 14 7 - - -1.87 Transcription
25 Phosphotransferase F2R313 26746/4.78 38 54 7 0 - +3.40 +2.82 Transport
26 Phosphoribosylaminoimidazole-succinocarboxamide synthase W9FWD8 33816/4.75 51 235 11 5 - -3.29 -2.13 Purine biosynthesis
27 Sulfurtransferase A0A087JZZ5 31,629/4.75 67 417 17 8 +1.98 +3.77 - Stress response
28 Cellulose-binding protein A0A087KFW0 34,734/5.28 62 333 21 7 - +4.44 +2.60 Cell wall biogenesis/degradation
29 Polyprenyl synthetase W9FVB1 36,453/5.29 51 121 11 2 - +2.31 +1.74 Isoprene biosynthesis
30 UDP-glucose 4-epimerase W9FYS0 34,585/5.33 54 175 13 3 - - +4.38 Carbohydrate metabolism
31 ATP-binding protein A0A087KCQ4 36,214/5.10 28 88 9 2 - +2.96 +1.84 Transport
32 Peptide chain release factor 2 B1VV08 40,843/4.70 54 284 15 6 - +3.30 +2.19 Protein biosynthesis/translation
33 Argininosuccinate synthase B1W3B3 43,579/4.94 35 250 13 4 - +1.99 +1.64 Arginine biosynthesis
34 Acetylornithine transaminase G0PPG1 45,532/5.79 35 209 11 4 - -2.40 - Arginine/lysine biosynthesis
35 Citrate synthase G0Q9J5 48,303/5.71 65 702 31 10 - -1.74 - Carbohydrate metabolism
36 Chorismate synthase B1W450 41,587/5.78 38 261 12 5 - +1.72 +2.15 Chorismate biosynthetic process
37 Glycogen phosphorylase B5GKX7 56,486/5.67 20 149 10 3 - -3.38 - Carbohydrate metabolism
38 Bifunctional protein GlmU B1VUI7 49,939/5.72 41 218 14 3 - +2.24 +2.11 LPS lipid A biosynthesis
39 Trigger factor B1VXA5 51,286/4.33 45 297 21 6 - +3.42 +3.86 Cell cycle/cell division/
40 30S ribosomal protein S1 A0A069JPK5 55,007/4.55 50 699 24 8 - +7.43 +6.70 Translation
41 30S ribosomal protein S1 B1W0V3 55,094/4.55 54 729 28 10 - +9.54 +5.67 Translation

MW, molecular weight; SC, the percentage of sequence coverage; MP, the number of matched peptides; MS, mass spectrometry; MS/MS, tandem MS.

a Protein score based on the combined MS and MS/MS spectra.

b Peptide hits as the unique number of MS/MS spectra that matched the trypsin peptide.

Editorial Office
Rm,904 (New Bldg.) The Korean Science & Technology Center 22,
Teheran-ro 7-Gil, Gangnamgu, Seoul 06130, Korea
Tel: +82-2-557-9360    Fax: +82-2-557-9361    E-mail:                

Copyright © 2021 by Korean Society of Plant Pathology.

Developed in M2PI

Close layer
prev next